Jose Manuel Baena
BRECA Health Care, Spain
Title: Design and characterization of bioinks with hyaluronic acid for tissue and bone-3D bioprinting
Biography
Biography: Jose Manuel Baena
Abstract
Statement of the Problem: The 3D bioprinting of tissues and organs represents a major breakthrough in regenerative medicine and tissue engineering. Cartilage and bone regeneration provides an alternative in the treatment of diseases such as degenerative osteoarthritis, injuries of articular cartilage, osteonecrosis and bone fractures, among others. The purpose of this study is to describe the design, development and preparation of a bioink with hyaluronic acid (HA) to manufacture cartilage and bone by 3D bioprinting.
Methodology & Theoretical Orientation: For the formulation of bioinks, two hyaluronic acids were studied: high molecular weight sodium hyaluronate (bioinkA) and low molecular weight sodium hyaluronate (bioinkB), both of intra-articular grade. The HA was combined with alginate and human chondrocytes. The biopaper studied was the polylactic acid (PLA). Cell viability was studied for each bioink.
Findings: The results obtained showed that the HA concentration before and after the bioprinting process did not affect chondrocyte viability. Additionally, cells remained in proliferation after 5 weeks. The rheological properties of each bioink showed mild differences between bioinkA and bioinkB.
Conclusion & Significance: Considering the mild differences in rheological properties between the two experimental bioinks, it may be concluded that both formulations can be used for cartilage and bone bioprinting.
Recent Publications:
1. Gálvez-Martín P, Hmadcha A, Soria B, Calpena-Campmany AC, Clares-Naveros B (2014) Study of the stability of packaging and storage conditions of human mesenchymal stem cell for intra-arterial clinical application in patient with critical limb ischemia. European Journal of Pharmaceutics and Biopharmaceutics 86:459-468.
2. Gálvez P, Clares B, Bermejo M, Hmadcha A, Soria B (2014) Standard requirement of a microbiological quality control program for the manufacture of human mesenchymal stem cells for clinical use. Stem Cells and Development 23:1074-1083.
3. Gálvez P, Martín MJ, Calpena AC, Tamayo JA, Ruiz MA, Clares B (2014) Enhancing effect of glucose microspheres in the viability of human mesenchymal stem cell suspensions for clinical administration. Pharmaceutical Research 31:3515-3528.
4. Martín MJ, Calpena AC, Fernández F, Mallandrich M, Gálvez P, Clares B (2015) Development of alginate microspheres as nystatin carriers for oral mucosa drug delivery. Carbohydrate Polymers 117:140-149.
5. Llavero F, Urzelai B, Osinalde N, Gálvez P, Lacerda HM, Parada LA, Zugaza JL (2015) Guanine nucleotide exchange factor αPIX leads to activation of the Rac 1GTPase/Glycogen phosphorylase pathway in interleukin (IL)-2-stimulated T cells. Journal of Biological Chemistry 290:9171-9182.