Miguel Angel Mateos Timoneda
CIBER of Bioengineering, Biomaterials and Nanomedicine, Spain
Title: Design of a Composite Bioink for Bioprinting Applications
Biography
Biography: Miguel Angel Mateos Timoneda
Abstract
3D bioprinting is an expanding field that allows the fabrication of customized tissue engineered scaffolds with encapsulated cells. Designing a biomaterial fit for 3D printing and cell encapsulation (bioink) is a complex task due to the high number of requirements that need to be accomplished. Bioinks are usually based on combinations of different hydrogels due to their encapsulation capacities, but other kinds of materials can be added in order to improve certain characteristics of the final scaffold. A novel composite bioink that includes alginate as a printable hydrogel and calcium-releasing particles as a vascularization promoter is optimized and studied. Rheometry studies show that the addition of calcium-releasing particles to alginate increases its viscosity, but does not alter its shear thinning properties; therefore maintaining the printability of the material. Solid scaffolds with theoretically high nutrient diffusion rates (filament diameter ≈ 200 µm) are printed using this novel bioink and a novel cross-linking method. A bioprinting procedure that includes encapsulation of cells is optimized and tried out successfully with different bioinks, obtaining good survival rates. The addition of calcium releasing-particles improves cell survival after the bioprinting process as well as during the first culture days. Moreover, the interaction between calcium-releasing particles and alginate is proven to be adequate for bioprinting and could be an interesting line of research for bone regeneration and tissue vascularization applications.
Recent Publications:
1. Levato R, Visser J, Planell JA, Engel E, Malda J, Mateosâ€Timoneda MA (2014) Biofabrication of tissue constructs by 3D bioprinting of cellâ€laden microcarriers. Biofabrication 6:035020.
2. Puñet X, Machauffé R, Giannotti MI, Rodríguezâ€Cabello JC, Sanz F, Engel E, Mateosâ€Timoneda MA, Planell JA (2013) Enhanced cell†material interactions through the biofunctionalization of polymeric surfaces with engineered peptides. Biomacromolecules 14:2690.
3. Levato R, Planell JA, Mateosâ€Timoneda MA, Engel E (2015) Role of ECM/peptide coatings on SDFâ€1a triggered mesenchymal stromal cell migration from microcarriers for cell therapy. Acta Biomater 18:59.
4. Baelo A, Levato R, Julián E, Crespo A, Astola J, Gavaldà J, Engel E, Mateosâ€Timoneda MA, Torrents E (2015) Disassembling bacterial extracellular matrix with DNaseâ€coated nanoparticles to enhance antibiotic delivery in biofilm infections. J Control Release 209:150.
5. Levato R, Mateosâ€Timoneda MA, Planell JA (2012) Preparation of biodegradable polylactide microparticles via a biocompatible procedure. Macromol Biosci 12:557.